| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 141 | Thermobifida fusca | TGGGTGGCGATCAACGAA | CGCTGACCGACTTGTGAGA | 59.26 | 59.71 | 157 | 125 |
100.00%
|
100.00%
|
| Primer 142 | Thermobifida fusca | AGTTCCTCAACGTGGACGC | ACGTGCGGATGATGTGCT | 60.30 | 59.73 | 153 | 125 |
100.00%
|
100.00%
|
| Primer 143 | Thermobifida fusca | GCGATGGTCAACACGCAGA | TGCTTTTCACCGTGCCCA | 61.03 | 60.12 | 247 | 125 |
100.00%
|
100.00%
|
| Primer 144 | Thermobifida fusca | AGCCGAATCCTGCCGATAC | AACCGCTTCTGCTGCCAT | 59.63 | 59.97 | 236 | 124 |
100.00%
|
100.00%
|
| Primer 145 | Thermobifida fusca | ACCACCACACTGAATGGCG | ACGTCTGCGAGGAAAGCA | 60.60 | 59.27 | 166 | 124 |
100.00%
|
100.00%
|
| Primer 146 | Thermobifida fusca | TGGCTGATTTTCGCGTGC | AACGAGTCCTGCGTGATGG | 59.44 | 60.08 | 206 | 124 |
100.00%
|
100.00%
|
| Primer 147 | Thermobifida fusca | GTTCAAGTGACGGACAGCG | TTCGACCGTGATGCGCTT | 59.15 | 60.05 | 288 | 124 |
100.00%
|
100.00%
|
| Primer 148 | Thermobifida fusca | GCGAACTGTTACGTCGTCG | ACTGCTCGTTGCCGAACA | 59.31 | 59.89 | 295 | 124 |
100.00%
|
100.00%
|
| Primer 149 | Thermobifida fusca | CGCCTCTTCCTCATGCAGT | TGAAAGAGCGCTGCACCA | 59.78 | 59.89 | 265 | 124 |
100.00%
|
100.00%
|
| Primer 150 | Thermobifida fusca | AGTCCGCACTGATCAACCG | AGGAACTGTGCCAGTGTCG | 60.08 | 59.93 | 210 | 124 |
100.00%
|
100.00%
|